DNA Clean up

DNA (PCR) Clean-up & Concentration

Genomic DNA & PCR Clean-up

• Highly efficient : 90 to 100% recoveries
• Sample Sources Quick (5 minute) spin column recovery of DNA from impure preparations of
  genomic DNA (e.g., Proteinase K digestions), plasmid DNA (including BAC), viral DNA, and
  whole genome amplified (wga) DNA. Can also be used for the purification of low molecular weight
  DNA (50 bp to 10 kb) from PCR, endonuclease digestion, post-RT cDNA synthesis, etc
• DNA Size Limits Capable of purifying small DNA fragments>50 bp and large sized DNAs up to 200 kb
• DNA Recovery Typically, up to 25 µg total DNA per column can be eluted into ≥35 µl of low salt
  DNA Elution Buffer or water.

DNA Recovery

Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb
the recovery is 95%. For DNA 11 kb to 23 kb the recovery is 90%.

Processing Time

15 Min

DNA Size Limits

50 bp to 23 kb

Sample Sources

DNA excised from TAE/TBE-buffered agarose gels.

DNA Purity

High quality, purified DNA is eluted with water which is especially well suited for sequencing,
ligation reactions, PCR, labeling, and restriction endonuclease digestions