| |
 |
 |
 |
 |
 |
 |
|
|
|
 |
|
|
| |
|
| |
 |
|
|
|
 |
|
|
 |
|
|
|
|
| |
|
|
| |
|
| |
|
|
|
| |
Probes |
|
|
| |
Dual-Labeled BHQ Probes are traditional, linear, FRET probes
incorporating a fluorophore and quencher covalently attached to
the 5' and 3' ends of an oligo typically 20 to 30 bases in length.
Fluorescence is released through the 5' exonuclease activity of Taq
polymerase, which cleaves off the fluorescent dye upon the probe's
hybridization to its complementary sequence. Dual-Labeled BHQ
Probes are ideal for detecting the presence and quantifying the
amount of specific target sequences.
|
|
NOTE:
Building Probes with an Internal Quencher (Currently available with
FAM only)
Adding an internal modification annotated by T(...) indicates that a
Thymidine base is attached to the internal modification.
Please be aware that an additional T base will be introduced into
the sequence when adding such an internal modification.
|
|
|
Building Probes with Wobble/Degenerate Bases
To ensure the most efficient processing of your oligo, use IUPAC code
when entering wobble/degenerate bases in the Sequence Entry field
instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.
|
|
Please be aware that sequences longer than 30 bases are unlikely to
have efficient quenching as an end-labeled probe. For such situations
please consider positioning the quencher internally instead.
Conversely, sequences shorter than 20 bases may have been
designed as a Minor Groove Binder (MGB™) probe and are unlikely
to bind at the proper temperature of 70 °C
|
|
 |
|
| |
|
| |
|
| |
|
| |
 |
 |
|
 |
Scale |
 |
|
 |
Item Name |
 |
|
 |
|
 |
|
| 50 nmol |
|
 |
ValuProbe, 5' FAM/3' BHQ-1 |
 |
|
 |
|
 |
|
| Dual-labeled Probe, 5' FAM/3' BHQ-1 |
100 nmol - 200 nmol - 1 µmol |
|
 |
 |
|
| Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3 |
200 nmol - 1 µmol |
|
 |
 |
|
| Dual-labeled Probe, 5' FAM/3' TAMRA |
100 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| 50 nmol -200 nmol - 1 µmol -25 nmol |
|
| Dual-labeled Probe, 5' TET/3' BHQ-1 |
|
 |
|
 |
|
| Dual-labeled Probe, 5' TET/3' TAMRA |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1 |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| Dual-labeled Probe, 5' JOE/3' BHQ-1 |
200 nmol - 1 µmol |
|
 |
 |
|
| Dual-labeled Probe, 5' HEX/3' BHQ-1 |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1 |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2 |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| Dual-labeled Probe, 5' TAMRA/3' BHQ-2 |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| Dual-labeled Probe, 5' ROX/3' BHQ-2 |
200 nmol - 1 µmol |
|
 |
 |
|
| Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2 |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2 |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| 50 nmol -200 nmol - 1 µmol |
|
| Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2 |
|
 |
|
 |
|
| Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650 |
200 nmol - 1 µmol |
|
 |
 |
|
| Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3 |
50 nmol -200 nmol - 1 µmol -25 nmol |
|
 |
 |
|
| Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2 |
50 nmol -200 nmol - 1 µmol |
|
 |
 |
|
Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3 |
50 nmol -200 nmol - 1 µmol |
|
 |
 |
|
| Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) |
50 nmol -200 nmol - 1 µmol |
|
 |
 |
|
| Primer Pair, (with probe purchase) Cartridge |
50 nmol -200 nmol - 1 µmol |
|
 |
 |
|
 |
|
 |
Purified (up to 30 bases each) |
 |
50 nmol -200 nmol - 1 µmol |
 |
|
 |
 |
|
| |
|
 |
|
| |
|
|
| |
|
|
 |
|
 |
|
|
|
 |
 |
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|