Microbial&Environmental-DNA-Purification

Microbial & Environmental DNA Purification

• Simple, efficient isolation of DNA (up to 25 µg/prep) from all types of tough-to-lyse fungi
(e.g., yeast) and bacteria in as little as 15 minutes.
• State-of-the-art, ultra-high density BashingBeads™ are fracture resistant and chemically inert.
• Can be used with any bead mill, disrupter, or vortex that can accommodate standard 2 ml tubes.
Omits the use of organic denaturants as well as proteinases.

Soil Microbe DNA MiniPrep™

Sources DNA is isolated from
bacteria, fungi, protozoa, and
algae in up to 0.25 g of soil or
from fungal/bacterial
cells directly.

Capable of recovering genomic
DNA up to and above 40 kb. In
most instances, mitochondrial
DNA and viral DNA (if present)
will also be recoveredt

Typically, up to 25 µg total DNA
is eluted into ≥ 25 µl DNA Elution
Buffer per sample.

Processing Time 15 min

DNA Kits

Fecal DNA Kits

Plant/Seed DNA Kits

Up to 25 μg total DNA is eluted into ≥ 25 μl DNA
Elution Buffer per sample.

DNA Recovery

Processing Time

15 min

Capable of recovering genomic DNA up to and
above 40 kb. In most instances, mitochondrial
DNA and viral DNA (if present) will also be recovered.

DNA Size Limits

Capable of recovering genomic DNA up to and above 40 kb.
In most instances, mitochondrial DNA and viral DNA (if present)
will also be recovered

Bacterial, protist, as well as host DNA can be
effectively isolated from a ≤150 mg sample of
mammalian feces

Sample Sources

Up to 150 mg that include leaves, stems, buds, flowers, fruit,
seeds, etc.

DNA Purity

High quality, inhibitor-free DNA is eluted with DNA
Elution Buffer suitable for the amplification of
bacterial, protist, and/or mammalian templates.
A260/A280 >1.8

High quality, inhibitor-free DNA is eluted with DNA Elution
Buffer that is suitable for PCR amplification. A260/A280 > 1.8

DNA Yield

Typically 20-80 ng DNA/mg plant material is recovered.