DNA

Bacterial Plasmid

• For mini-, midi-, and maxi-scale preparations
• innovative colored buffers included in the kit permit error-free visualization and identification
  of complete bacterial cell lysis and neutralization.
• pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and
  resuspension steps common to classical plasmid preparation procedures
• High yields and concentration of purified DNA Up to 10 mg depending on the plasmid copy
  number, culture growth conditions, and strain of E. Coli utilized.
• free of endotoxins plasmid DNA suitable for transfection of sensitive cells.
• Processing Time 8 min
• Plasmid DNA Size Up to 25 kb

Yeast Plasmid

Free of endotoxins plasmid
DNA suitable for transfection
of sensitive cells

Ideal for low copy and
hard-to-isolate plasmids.

Format Isopropanol
Precipitation and Spin column

Plasmid DNA Size DNA
up to 23 kb.

Compatibility S. cerevisiae, C. albicans and S. pombe, and other
fungi species sensitive to yeast lytic enzymatic digestion

Processing Time 15 min

DNA Purity High-purity and endotoxin-free (<50 EU/μg) plasmid
DNA in low salt buffer or water; typical A260/280≥ 1.8.

Plasmid DNA Size Up to ~200 kb.

Plasmid DNA Yield Up to 10 μg per preparation, depending on the
plasmid copy number, initial volume of E. Coli culture processed,
and culture growth conditions. The typical yield is 1 μg per 3 ml of
.

Recovery Volume ≥ 10 µl