PCR

Routine PCR Enzymes & Master Mixes

we offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative,
high quality tools for DNA amplification and PCR. We offer a broad variety of DNA and RNA polymerases, nucleotide
mixes, kits, master mixes and more.

• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• High sensitivity and amplicon length
• Direct loading of PCR products on gels
• Novel buffer system - optimized and including dNTPs and MgCl2
• Robust - high-yield across a wide range of templates
• Red dye - for direct gel loading
• Convenient - all-in-one mastermix

Hot-start polymerases

• All-in-one mastermix formulations - for enhanced reproducibility
• Fast - complete PCR reaction in less than 30 minutes
• Antibody-based hot-start polymerase - for highest specificity
• Superior performance - highly optimized buffer system, including ultra-pure dNTPs and MgCl2
• dye - for direct gel loading
• Higher yields and amplicons length than with any hot start Taq (up to ~6 kb genomic)
• Minimal reaction optimization due to high inhibitor tolerance

Multiplex PCR Enzymes & Master Mixes

Multiplex PCR Master Mix is an optimization-free multiplex PCR master mix designed specifically for endpoint multiplex PCR. It allows you to easily
perform multiplexing with minimal optimization. Coupled with its proven performance over wide range of amplicon sizes from 50 bp to 2.5 kb, it will
greatly enhance your workflow flexibility.

• Simultaneous amplification of over 20 targets up to 2.5 kb in length
• Excellent results even with GC-rich or suboptimal purity templates
• Minimal optimization of reaction conditions required
• Direct loading of PCR products on gels with Green Buffer formats
• All-in-one mastermix formulations - for enhanced reproducibility
• Fast - complete PCR reaction in less than 30 minutes
• Dye - for direct gel loading
• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Successful multiplex PCR saves time and reagents
• Higher yields and amplicons length than with any hot start Taq (up to ~6 kb genomic)

High-Fidelity Polymerases

• Highest fidelity amplification (>100X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• High Yield - Increased product yield using minimal amount of enzyme.
• Hot start and master mix formats available
• High-processivity - for longer amplicons
• Fast amplification - for short run times
• Ideal for a wide range of templates - including long templates greater than 5kb
• Eight times more accurate than Taq DNA polymerase
• Highly thermostable —remains 95% active after 2 hours incubation at 95°C
• Generates blunt-end PCR products

Direct PCR Enzymes & Master Mixes:

Direct PCR approach delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified
samples. Significant savings in time and cost can be achieved in applications such as plant and animal genotyping

• Direct PCR—sample is added directly to PCR reaction, therefore therese is no need for
time-consuming and expensive DNA purification steps
• Rapid extraction protocol - high-yield, PCR-ready DNA in 15 minutes
• Easy-to-use - Two simple protocols for various applications
• Convenient format - single-tube extraction minimizes contamination risks
• Master Mix format with premixed gel loading dye—minimizes possibility of
cross-contamination, reduces sample handling and allows direct loading on gel

Human Direct PCR kits

Animal Direct PCR kits

Blood Direct PCR kits

Plant Direct PCR kits